Sheep Complement Component 4 (C4) ELISA Kit

Cat.#:

JL47505

Regulatory Status:

RUO

Alternative:

Complement Component 4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

3.12-200ng/mL

Sensitivity:

1.51ng/mL

Specificity:

This kit recognizes Sheep C4 in samples. No significant cross-reactivity or interference between Sheep C4 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| C4

Complement component 4 (C4), is a protein involved in the intricate complement system, originating from the human leukocyte antigen (HLA) system. It serves a number of critical functions in immunity, tolerance, and autoimmunity with the other numerous components. Furthermore, it is a crucial factor in connecting the recognition pathways of the overall system instigated by antibody-antigen (Ab-Ag) complexes to the other effector proteins of the innate immune response. For example, the severity of a dysfunctional complement system can lead to fatal diseases and infections. Complex variations of it can also lead to schizophrenia.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Sheep C4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	82-101	94
Plasma(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Sheep C4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-96	88-96
1 : 2	Average (%)	92	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98